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... all the preparations for the surgery and activate the refrigeration canister, retrieving a small cluster of the First's cellular tissue from within.

This small cluster of the First's cellular tissue was something Shinji had secretly obtained through Yakumo.

After the First's passing, the Second, using the most advanced technological means, preserved a large amount of the First's cellular tissue. This particular sample was one of those preserved by the Second at the time.

...

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