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... Construct SgRNA-Cas9 vectors.
Build SgRNA-Cas9 stable strains.
… and so on.
Can Su Hao accomplish it? b𝚎dn𝚘v𝚎𝚕.𝚘𝚛𝚐
The answer is no!
Conditions do not allow for it, and he may encounter any adverse situation in the future; this method of synthesizing Cas9 is not suitable for him.
What he needs is a simple and straightforward method, one that can be used anywhere to synthesize.
Moreover, he already has an idea, and this idea is likely ...
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